MOE Workshop Series

North America

September 14, 2017

Location: Sheraton La Jolla Hotel
3299 Holiday Court, San Diego, CA, 92037, United States
Complimentary continental breakfast and lunch will be provided.

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 Antibody Modeling and Protein Engineering in MOE
The course covers approaches for structure-based antibody design and includes protein-protein interactions analysis, in silico protein engineering, affinity modeling and antibody homology modeling. The interaction of a co-crystallized antibody-antigen complex will be studied by generating and examining the molecular surfaces and visualizing protein-protein interactions in 3D and 2D. Antibody properties will be evaluated using specialized calculated protein property descriptors and analyzing protein patches. The application of protein engineering tools for homology modeling and conducting property optimization of antibodies in the context of developability will be studied. Antibody optimization examples will include identification of glycosylation sites and analysis of correlated pairs using a specialized antibody database. An approach for humanizing antibody homology models will be discussed. All the steps necessary for producing and assessing antibody homology models will be described.

 Protein Alignments, Superpositions, Homology Modeling and Loop Modeling
The course covers protein alignments and homology modeling in MOE. The homology modeling section covers the complete sequence to structure workflow, including template searching, query alignment, adjustment of the alignment between the sequence and the template, homology model building and refinement, along with applications for assessing model quality. Refinement of protein loops and loop conformations using the Loop Modeler and LowModeMD conformational searching will be discussed. Protein contacts will be evaluated after alignment and superposition of protein multimers. A method for superposing proteins that is strictly based on structural motifs will be described.

For questions, please contact:
Raul Alvarez
Senior Marketing Manager