UGM & Conference in North America

Structural analysis of antibody-antigen complexes by crystallography and modelling

Rafael Depetris
Principal Scientist, Kadmon Pharmaceuticals
(FRIDAY, June 23 - Scientific Presentations, 15:00-15:30)

X-Ray crystallography is a very accurate tool to determine the structure of small and large proteins and protein complexes at high resolution. However, obtaining good quality crystals can be a bottleneck that undermines even the most determined efforts. Building models of the target proteins and complexes is an extremely helpful step for this purpose. It can not only produce preliminary information but also help in further protein engineering. Nevertheless, a careful approach is needed during the modeling process in order to establish the selection of binding site and set up proper validation. We will present an example and show how generating models helps to know better the proteins that one is dealing with.